Not known Facts About attune flow cytometer
Not known Facts About attune flow cytometer
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Details-enriched characterization of mobile populations. Morphological information and facts from visuals can insert to the richness of flow cytometry information. For example, the determine reveals an normally common apoptosis assay utilizing Annexin V and PI, introducing cell imaging to characterize cells in each populace to expose morphologically unique functions. These insights could not are received from multiplex staining alone.
As samples are obtained to the Attune CytPix Flow Cytometer, the higher-speed brightfield digicam captures and suppliers photos of detected gatherings at a amount around six,000 photos per 2nd, according to the flow price and impression measurement. For greater flexibility, Attune Cytometric Software package lets you modify the image seize frequency as desired and choose a specific gate for graphic seize, if needed.
Incorporate acoustic focusing technologies with substantial-parameter Assessment for adaptable spectral unmixing and conventional workflows.
Learn new insights into your experimental facts by visualizing populations working with blended fluorescence and morphology data. Our computer software interface wizards empower downsampling, concatenation (merged files) and dimensionality resources which can be developed in to the software program for an intuitive, streamlined facts Examination working experience.
"An image is not very worth one thousand dots, nevertheless it seriously really helps to see visuals. It is excellent to discover visuals that correlate with staining. For example, AnnexinV-positive cells which might be morphologically different than healthier cells, or CD14+ monocytes which are more substantial and even more textured than CD3+/CD4+/CD14- lymphocytes.
Listed here, a highly trained user has gated singlets confidently. Soon after evaluating the manual singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate has more than four% aggregates.
Pair your Attune flow cytometer using an autosampler for greater workflow efficiency, and insert robotic integration for maximum throughput.
Regular image quality even at higher throughput Acoustic concentrating and also a superior-speed digicam Merge to impression these T cells regularly at small or significant flow prices. Conveniently change concentration and digicam options to satisfy experimental prerequisites.
Early signs of this contamination had initially been dismissed as particles. Backgating to discover new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also enables you to use morphological functions to find intriguing subpopulations that might not be evident from flow cytometry info on your own.
Consumers can look at event images in authentic-time to verify sample excellent, optimize protocols, and report the morphology of each and every mobile populace recognized for Assessment. Imaging capture also Positive aspects from acoustic concentrating, which really helps to posture the cells to ensure that a pointy, centered image is received.
Seem waves permit specific mobile alignment, leading to Outstanding information excellent and ~10X more quickly sample flow charges.
We Earlier demonstrated the power of imaging Motor vehicle-T/Ramos cell interactions. Allow’s evaluate just the populace of biggest fascination, the double constructive functions, To find out more. We can now use extended morphological parameters (below, circularity and skewness of depth) to further take a look at the features of these populations and refine gating on these functions, escalating data robustness.
Leverage the strength of State-of-the-art technological innovation and innovation to accelerate your discoveries. Rely on during the Attune Flow Cytometer relatives to deliver the reliability, speed, and accuracy that established your perform aside.
No wash/no lyse leukocyte Investigation with imaging. Cells were acquired from 24-hour-old blood by dilution in one mM EDTA ( Specialist UX/UI permits people to practice AI types for expanded apps. (A) The “Coach Product” aspect gives annotation resources and genuine-time design teaching accuracy.
Gating on these extended parameters means that you can swiftly and properly discover populations of read more curiosity and confirm gating method with little if any manual evaluate. The Attune computer software offers use of state-of-the-artwork machine Understanding Assessment applications in an expertly intended person interface, rendering it exceptionally convenient to use.
The Attune Xenith cytometer can be a substantial-parameter spectral analyzer, offering both equally spectral unmixing and conventional flow capabilities for comprehensive and adaptable cell analysis.
The Attune CytPix Flow Cytometer enables buyers to visualize samples all through acquisition, validate the precision of gating techniques, and support results with reputable documentation for publication reasons.
Listed here, we exhibit that by gating strategies using graphic-based quantitative parameters can distinguish interacting cells from coincident events to extra accurately evaluate interacting cells.